RS/tRNA Usage Publications
Hill, Lindsay K., Joseph A. Frezzo, Priya Katyal, Dung Minh Hoang, Zakia Ben Youss Gironda, Cynthia Xu, Xuan Xie, Erika Delgado-Fukushima, Youssef Z. Wadghiri, and Jin Kim Montclare. (mar) 2019. “Protein-Engineered Nanoscale Micelles For Dynamic 19F Magnetic Resonance And Therapeutic Drug Delivery”. Acs Nano 13: 2969-2985. doi:10.1021/acsnano.8b07481.
RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
N6-Trifluoroacetyl-L-lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
They are used for sites specifically studying post-translational modification acetylation events on particular lysine residues. Also be used as an F19 NMR probe.
RS Organism of Origin
Parent RS
RS Mutations
L266M
L270I
L274A
C313F
L270I
L274A
C313F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
N/A
Multiple tRNAs?
N/A
RS/tRNA Availability
It is not Commercially available but can be made by applying the mutation to Mb-PylRS/tRNA.
RS/tRNA Additional Notes
This RS is less efficient at incorporating TFAcK compared to AcKRS2 at 5mM in sfGFP in E.coli, when measuring Fluorescence. Use AcKRS2 for TFAcK incorporation.