RS/tRNA Pair Development Year
2015
RS Organism of Origin
Parent RS
RS Mutations
E107T
F108Y
Q109M
F108Y
Q109M
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
R257G
Multiple tRNAs?
N/A
RS/tRNA Availability
Can be procured from AddGene: Plasmid #73546
Used in what cell line?
RS/tRNA Additional Notes
This RS/tRNA pair was evolved with MAGE[ multiplex automated genome engineering]. The evolved pair has been shown to have 25 fold higher expression of pAzF in GFP[3UAG] compared to the progenitor enzyme (pAcFRS). This pair has also been shown to be promiscuios, and specifically also incorporates pAcF.