RS/tRNA Foundational Publication Support
Amiram, Miriam, Adrian D. Haimovich, Chenguang Fan, Yane-Shih Wang, Hans-Rudolf Aerni, Ioanna Ntai, Daniel W. Moonan, et al. (dec) 2015. “Evolution Of Translation Machinery In Recoded Bacteria Enables Multi-Site Incorporation Of Nonstandard Amino Acids”. Nature Biotechnology 33: 1272-1279. doi:10.1038/nbt.3372.
RS/tRNA Usage Publications
Castro, Ana, Juan Manuel Carreño, James Duehr, Florian Krammer, and Ravi S. Kane. (aug) 2021. “Refocusing The Immune Response To Selected Epitopes On A Zika Virus Protein Antigen By Nanopatterning”. Advanced Healthcare Materials 10: e2002140. doi:10.1002/adhm.202002140.
Shapiro, Daniel Mark, Gunasheil Mandava, Sibel Ebru Yalcin, Pol Arranz-Gibert, Peter J. Dahl, Catharine Shipps, Yangqi Gu, et al. (feb) 2022. “Protein Nanowires With Tunable Functionality And Programmable Self-Assembly Using Sequence-Controlled Synthesis”. Nature Communications 13: 829. doi:10.1038/s41467-022-28206-x.
Stadler, Karina A., Walter Becker, Barbara Darnhofer, Ruth Birner-Gruenberger, and Klaus Zangger. (jul) 2022. “Overexpression Of Recombinant Proteins Containing Non-Canonical Amino Acids In Vibrio Natriegens: P-Azido-L-Phenylalanine As Coupling Site For 19F-Tags”. Amino Acids 54: 1041-1053. doi:10.1007/s00726-022-03148-2.
Li, Allyson, Rashmi Voleti, Minhee Lee, Dejan Gagoski, and Neel H. Shah. (mar) 2023. “High-Throughput Profiling Of Sequence Recognition By Tyrosine Kinases And Sh2 Domains Using Bacterial Peptide Display”. Elife 12: e82345. doi:10.7554/eLife.82345.
RS/tRNA Pair Development Year
2015
ncAA(s) Incorporated
p-azido-L-phenylalanine (pAzF)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Used as a photocrosslinker, allowing for crosslinking and bioorthogonal ligation of protein.
RS Organism of Origin
Parent RS
RS Mutations
E107T
F108Y
Q109M
F108Y
Q109M
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
R257G
Multiple tRNAs?
N/A
RS/tRNA Availability
Can be procured from AddGene: Plasmid #73546
Used in what cell line?
RS/tRNA Additional Notes
This RS/tRNA pair was evolved with MAGE[ multiplex automated genome engineering]. The evolved pair has been shown to have 25 fold higher expression of pAzF in GFP[3UAG] compared to the progenitor enzyme (pAcFRS). This pair has also been shown to be promiscuios, and specifically also incorporates pAcF.