RS/tRNA Foundational Publication Support
Chin, Jason W., Andrew B. Martin, David S. King, Lei Wang, and Peter G. Schultz. (aug) 2002. “Addition Of A Photocrosslinking Amino Acid To The Genetic Code Of Escherichia Coli”. Proceedings Of The National Academy Of Sciences 99: 11020-11024. doi:10.1073/pnas.172226299.
Sato, Shin, Shinya Mimasu, Aya Sato, Nobumasa Hino, Kensaku Sakamoto, Takashi Umehara, and Shigeyuki Yokoyama. (2011) 2011. “Crystallographic Study Of A Site-Specifically Cross-Linked Protein Complex With A Genetically Incorporated Photoreactive Amino Acid.”. Biochemistry 50 (2): 250-7.
RS/tRNA Usage Publications
Nelson, Brock, Nathan Soper, and Tania J. Lupoli. (oct) 2023. “Bacterial J-Domains With C-Terminal Tags Contact The Substrate Binding Domain Of Dnak And Sequester Chaperone Activity”. Chembiochem: A European Journal Of Chemical Biology 24: e202300261. doi:10.1002/cbic.202300261.
Tholen, Marrit M. E., Bas J. H. M. Rosier, Robin T. Vermathen, Céline A. N. Sewnath, Cornelis Storm, Laura Woythe, Cristina Izquierdo-Lozano, et al. (jun) 2023. “Mapping Antibody Domain Exposure On Nanoparticle Surfaces Using Dna-Paint”. Acs Nano 17: 11665-11678. doi:10.1021/acsnano.3c02195.
Blueggel, Mike, Alexander Kroening, Matthias Kracht, Johannes van den Boom, Matthias Dabisch, Anna Goehring, Farnusch Kaschani, et al. (jun) 2023. “The Ubx Domain In Ubxd1 Organizes Ubiquitin Binding At The C-Terminus Of The Vcp/P97 Aaa-Atpase”. Nature Communications 14: 3258. doi:10.1038/s41467-023-38604-4.
van den Boom, Johannes, Guendalina Marini, Hemmo Meyer, and Helen R. Saibil. (jul) 2023. “Structural Basis Of Ubiquitin-Independent Pp1 Complex Disassembly By P97”. The Embo Journal 42: e113110. doi:10.15252/embj.2022113110.
van Aalen, Eva A., Bas J. H. M. Rosier, Tom Jansen, Simone F. A. Wouters, Robin T. Vermathen, Harmen J. van der Veer, José Yeste Lozano, et al. (jun) 2023. “Integrated Bioluminescent Immunoassays For High-Throughput Sampling And Continuous Monitoring Of Cytokines”. Analytical Chemistry 95: 8922-8931. doi:10.1021/acs.analchem.3c00745.
RS/tRNA Pair Development Year
2002
ncAA(s) Incorporated
p-benzoyl-L-phenylalanine (Bpa)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Used as a photocrosslinker, allowing for crosslinking and bioorthogonal ligation of protein.
RS Organism of Origin
Parent RS
RS Mutations
Y32G
E107S
D158T
I159S
E107S
D158T
I159S
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
N/A
Multiple tRNAs?
N/A
RS/tRNA Availability
available in pEVOL plasmid as AddGene #31190
Also from 2011 foundational paper as Addgene plasmid #197102 (contains an alternate tRNA with bases 45G and 47U) and lists a 286R mutation in the BpaRS.
Also from 2011 foundational paper as Addgene plasmid #197102 (contains an alternate tRNA with bases 45G and 47U) and lists a 286R mutation in the BpaRS.
RS/tRNA Additional Notes
This is currently the most used RS/tRNA pair for pBpF incorporation.