ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Jackson, Jennifer C, Jared T Hammill, and Ryan A Mehl. (2007) 2007. “Site-Specific Incorporation Of A (19)F-Amino Acid Into Proteins As An Nmr Probe For Characterizing Protein Structure And Reactivity.”. Journal Of The American Chemical Society 129 (5): 1160-6.
Augustin, George, Fatema Bhinderwala, Nathan D Alexander, Iker Hernández, Stanislau Stanisheuski, Christina M Monnie, Alex J Eddins, et al. (2026) 2026. “Endogenous Site-Specific Encoding Of Trifluoromethyl-Bearing Phenylalanine And Tryptophan For In-Cell F Nmr.”. Journal Of The American Chemical Society 148 (7): 6744-6749. doi:10.1021/jacs.5c18349.
ncAA Usage Publications
Augustin, George, Fatema Bhinderwala, Nathan D Alexander, Iker Hernández, Stanislau Stanisheuski, Christina M Monnie, Alex J Eddins, et al. (2026) 2026. “Endogenous Site-Specific Encoding Of Trifluoromethyl-Bearing Phenylalanine And Tryptophan For In-Cell F Nmr.”. Journal Of The American Chemical Society 148 (7): 6744-6749. doi:10.1021/jacs.5c18349.
ncAA Utility
19F Probe for protein
NMR
NMR
ncAA Source
Exogenous - Purchased
ncAA Availability
Can be purchased from https://www.thermofisher.com/order/catalog/product/H52170.03
RS/tRNA Pair Usage Information
2007 foundational paper generated tfm-PheRS for use in E. coli.
2026 foundational paper showed the fairly permissive EAziRS efficiently installed this ncAA in mammalian cells.
2026 foundational paper showed the fairly permissive EAziRS efficiently installed this ncAA in mammalian cells.
ncAA Synonyms
4-(Trifluoromethyl)-L-phenylalanine
114926-38-4
(2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid
L-Phenylalanine, 4-(trifluoromethyl)-
122839-56-9
tfmPhe
114926-38-4
(2S)-2-amino-3-[4-(trifluoromethyl)phenyl]propanoic acid
L-Phenylalanine, 4-(trifluoromethyl)-
122839-56-9
tfmPhe
ChEBI ID
232918
PubChem Link