ncAA Structure (png, jpg, jpeg)
![N6-[(2-(3-Methyl-3H-diazirin-3-yl)ethoxy)carbonyl]-L-lysine](/sites/gce4all/files/styles/medium/public/2025-01/Page_20_Structure_6.png?itok=YosSmxRs)
ncAA Foundational Publication Support
Ai, Hui-Wang, Weijun Shen, Amit Sagi, Peng R Chen, and Peter G Schultz. (2011) 2011. “Probing Protein-Protein Interactions With A Genetically Encoded Photo-Crosslinking Amino Acid.”. Chembiochem : A European Journal Of Chemical Biology 12 (12): 1854-7. doi:10.1002/cbic.201100194.
“"Genetically Encoding An Aliphatic Diazirine For Protein Photocrosslinking"”. "Chem. Sci." "2". "The Royal Society of Chemistry": 480-483. doi:"10.1039/C0SC00373E".
Hancock, S. M., R. Uprety, A. Deiters, and J. W. Chin. n.d. “Expanding The Genetic Code Of Yeast For Incorporation Of Diverse Unnatural Amino Acids Via A Pyrrolysyl-Trna Synthetase/Trna Pair” 132: 14819+.
ncAA Utility
Introduced as a photocrosslinking agent that was aliphatic rather than aromatic giving it more flexibility. Similar in structure to DiZPK.
ncAA Source
Exogenous - Synthesized
ncAA Availability
Synthetic routes are reported in the 2011 foundational paper and in the DOI: 10.1039/c0sc00373e paper
RS/tRNA Pair Usage Information
In the absence of a head-to-head comparison, it is unclear how the two RSs compare. The ABK RS is available on Addgene and seems to be used more often. The second foundational paper reported using WT MbPylRS for this ncAA and the third showed it could be used in yeast.
ncAA Synonyms
Nε-[(2-(3-Methyl-3H-diazirin-3-yl)ethoxy)carbonyl]-L-lysine
DiAzKs
(S)-2-amino-6-((2-(3-methyl-3H-diazirin-3-yl)ethoxy)carbonylamino)hexanoic acid hydrochloride
(2S)-2-amino-6-[2-(3-methyldiazirin-3-yl)ethoxycarbonylamino]hexanoic acid
(2S)-2-amino-6-({[2-(3-methyl-3H-diazirin-3-yl)ethoxy]carbonyl}amino)hexanoic acid
H-L-Photo-Lysine*HCl
SCHEMBL875173
HY-D0853
3'-azibutyl-N-carbamoyl lysine
ABK
AbK
DiAzKs
(S)-2-amino-6-((2-(3-methyl-3H-diazirin-3-yl)ethoxy)carbonylamino)hexanoic acid hydrochloride
(2S)-2-amino-6-[2-(3-methyldiazirin-3-yl)ethoxycarbonylamino]hexanoic acid
(2S)-2-amino-6-({[2-(3-methyl-3H-diazirin-3-yl)ethoxy]carbonyl}amino)hexanoic acid
H-L-Photo-Lysine*HCl
SCHEMBL875173
HY-D0853
3'-azibutyl-N-carbamoyl lysine
ABK
AbK
ChEBI ID
233057
PubChem Link