RS/tRNA Foundational Publication Support
Lee, Hyun Soo, Jiantao Guo, Edward A Lemke, Romerson D Dimla, and Peter G Schultz. (2009) 2009. “Genetic Incorporation Of A Small, Environmentally Sensitive, Fluorescent Probe Into Proteins In Saccharomyces Cerevisiae.”. Journal Of The American Chemical Society 131 (36): 12921-3. doi:10.1021/ja904896s.
RS/tRNA Pair Development Year
2009
ncAA(s) Incorporated
3-(Naphthalen-2 ylamino)-2 aminopropanoic acid (Nap)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
RS Organism of Origin
Parent RS
RS Mutations
L38F
M40G
L41P
Y499V
Y500L
Y527A
H537G5
L538S
F541C
A560V
M40G
L41P
Y499V
Y500L
Y527A
H537G5
L538S
F541C
A560V
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
Incorporated Anap into hSOD-33TAG at 1.2mg/L in shake flasks.
Able to incorporate Glutamine Binding Protein(QBP) at amber codon position Asn160. Amber mutant QBP containing a C-terminal His6 tag (QBP-160TAG) was expressed and purified in the presence of the evolved tRNA Leu CUA /Anap-2C pair and 0.4 mM Anap in S. cerevisiae.
Incorporation of Anap into QBP was confirmed by ESI-MS
Able to incorporate Glutamine Binding Protein(QBP) at amber codon position Asn160. Amber mutant QBP containing a C-terminal His6 tag (QBP-160TAG) was expressed and purified in the presence of the evolved tRNA Leu CUA /Anap-2C pair and 0.4 mM Anap in S. cerevisiae.
Incorporation of Anap into QBP was confirmed by ESI-MS