RS/tRNA Foundational Publication Support
Erdmann, Ines, Kathrin Marter, Oliver Kobler, Sven Niehues, Julia Abele, Anke Müller, Julia Bussmann, et al. (2015) 2015. “Cell-Selective Labelling Of Proteomes In Drosophila Melanogaster.”. Nature Communications 6: 7521. doi:10.1038/ncomms8521.
Mahdavi, Alborz, Graham D Hamblin, Granton A Jindal, John D Bagert, Cathy Dong, Michael J Sweredoski, Sonja Hess, Erin M Schuman, and David A Tirrell. (2016) 2016. “Engineered Aminoacyl-Trna Synthetase For Cell-Selective Analysis Of Mammalian Protein Synthesis.”. Journal Of The American Chemical Society 138 (13): 4278-81. doi:10.1021/jacs.5b08980.
RS/tRNA Pair Development Year
2015
ncAA(s) Incorporated
Azidonorleucine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Biorthogonal Ligation, Click Chemistry labeling with fluorescence or affinity labels. Specifically used for cell-specific proteomics studies using "bioorthogonal noncanonical amino acid tagging" (BONCAT) proteomics. Contrasts with Azidohomoalanine which does not allow cell-specific studies.
RS Organism of Origin
Parent RS
RS Mutations
L274G
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
UAC
RS/tRNA Availability
Can be obtained from AddGene Plasmid #63177
Also, as Plasmid #89189 (contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS)
Also, as Plasmid #89189 (contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS)
RS/tRNA Additional Notes
2015 study showed this RS works in live drosophila. 2016 study showed robust Anl incorporation in CHO, COS7, and HeLa cells by TAMRA labeling, even while the RS activates Met 4-fold faster than Anl at equal concentrations.