RS/tRNA Foundational Publication Support
Yuet, Kai P, Meenakshi Doma, John T Ngo, Michael J Sweredoski, Robert L J Graham, Annie Moradian, Sonja Hess, Erin M Schuman, Paul Sternberg, and David A Tirrell. (2015) 2015. “Cell-Specific Proteomic Analysis In Caenorhabditis Elegans.”. Proceedings Of The National Academy Of Sciences Of The United States Of America 112 (9): 2705-10. doi:10.1073/pnas.1421567112.
RS/tRNA Pair Development Year
2015
ncAA(s) Incorporated
p-azido-L-phenylalanine (pAzF)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Used as a photocrosslinker, allowing for crosslinking and bioorthogonal ligation of protein.
RS Organism of Origin
Parent RS
RS Mutations
T412G
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
wildtype anticodons
RS/tRNA Availability
n/a
Used in what cell line?
RS/tRNA Additional Notes
Works ~20-fold better on pAzF than Phe. Used for whole organism and tissue specific "BONCAT" proteomics studies in live C elegans. pAzF was not taken up by C elegans from the media, but was provided by feeding the worms with an E coli Phe auxotroph (KY14) that had Thr251GlyEcRS (the E coli equivalent of the Ce RS mutant) to incorporate pAzF replacing Phe at an ~50% level. Also showed equivalent Thr->Gly mutant in yeast, drosophila, zebrafish, and mouse PheRS led to incorporation of pAzF. The change in the human PheRS functioned only very little.