ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Zhang, Michael Shaofei, Simon F Brunner, Nicolas Huguenin-Dezot, Alexandria D Liang, Wolfgang H Schmied, Daniel T Rogerson, and Jason W Chin. (2017) 2017. “Biosynthesis And Genetic Encoding Of Phosphothreonine Through Parallel Selection And Deep Sequencing.”. Nature Methods 14 (7): 729-736. doi:10.1038/nmeth.4302.
ncAA Utility
Used to produce site-specifically phosphorylated proteins at a threonine residue via genetic code expansion versus other protein phosphorylation strategies.
ncAA Source
Endogenous - Biosynthesized
ncAA Availability
You can express the pduX gene (from Salmonella enterica) in E. coli to produce intracellular pThr (as noted in "Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing."). This gene can be found on the same plasmid for pThrRS from Addgene (#173899).
ncAA Synonyms
(2S,3R)-2-amino-3-hydroxybutanoic acid 3-phosphate
O-phospho-L-threonine
O-phosphono-L-threonine
pThr
L-phosphothreonine
O-phospho-L-threonine
O-phosphono-L-threonine
pThr
L-phosphothreonine
ChEBI ID
37525
PubChem Link
PDB Code
TPO