ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Zhu, Phillip, Philip R Gafken, Ryan A Mehl, and Richard B Cooley. (2019) 2019. “A Highly Versatile Expression System For The Production Of Multiply Phosphorylated Proteins.”. Acs Chemical Biology 14 (7): 1564-1572. doi:10.1021/acschembio.9b00307.
ncAA Usage Publications
Stuber, Katrin, Tobias Schneider, Jill Werner, Michael Kovermann, Andreas Marx, and Martin Scheffner. (2021) 2021. “Structural And Functional Consequences Of Nedd8 Phosphorylation.”. Nature Communications 12 (1): 5939. doi:10.1038/s41467-021-26189-9.
ncAA Utility
Used to produce site-specifically phosphorylated proteins at a serine residue via genetic code expansion versus other protein phosphorylation strategies.
ncAA Source
Endogenous - Biosynthesized
ncAA Availability
pSer is the natural precursor to Serine, and by knocking out SerB (last step in Ser production pathway), intracellular pSer builds up and can be used for GCE.
SepRS2/Sep-tRNA(B4) submission shows where to obtain appropriate cell lines.
SepRS2/Sep-tRNA(B4) submission shows where to obtain appropriate cell lines.
ncAA Synonyms
(2S)-2-amino-3-(phosphonooxy)propanoic acid
O-phosphoserine
pSer
O-phospho-L-serine
O-phosphono-L-serine
O-phosphoserine
pSer
O-phospho-L-serine
O-phosphono-L-serine
ChEBI ID
15811
PubChem Link
PDB Code
SEP