ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Luo, Xiaozhou, Guangsen Fu, Rongsheng E Wang, Xueyong Zhu, Claudio Zambaldo, Renhe Liu, Tao Liu, et al. (2017) 2017. “Genetically Encoding Phosphotyrosine And Its Nonhydrolyzable Analog In Bacteria.”. Nature Chemical Biology 13 (8): 845-849. doi:10.1038/nchembio.2405.
ncAA Utility
Used to produce site-specifically phosphorylated proteins at a tyrosine residue via genetic code expansion versus other protein phosphorylation strategies.
ncAA Source
Exogenous - Synthesized
ncAA Availability
Bioavailability of intracellular pTyr (or its non-hydrolyzable mimic Pmp) can be increased by supplementing media with the dipeptide Lys-pTyr (or Lys-Pmp). The dipeptide is synthesized according to the foundational paper referenced:
Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria (Luo et al., 2017).
Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria (Luo et al., 2017).
ncAA Synonyms
O-phosphotyrosine
O-phospho-L-tyrosine
O4-phospho-L-tyrosine
O-phosphono-L-tyrosine
phosphonotyrosine
(2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid
O-phospho-L-tyrosine
O4-phospho-L-tyrosine
O-phosphono-L-tyrosine
phosphonotyrosine
(2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid
ChEBI ID
37788
PubChem Link
PDB Code
PTR