RS/tRNA Foundational Publication Support
Xie, Jianming, Lubica Supekova, and Peter G Schultz. (2007) 2007. “A Genetically Encoded Metabolically Stable Analogue Of Phosphotyrosine In Escherichia Coli.”. Acs Chemical Biology 2 (7): 474-8.
Luo, Xiaozhou, Guangsen Fu, Rongsheng E Wang, Xueyong Zhu, Claudio Zambaldo, Renhe Liu, Tao Liu, et al. (2017) 2017. “Genetically Encoding Phosphotyrosine And Its Nonhydrolyzable Analog In Bacteria.”. Nature Chemical Biology 13 (8): 845-849. doi:10.1038/nchembio.2405.
RS/tRNA Pair Development Year
2007
ncAA(s) Incorporated
phosphotyrosine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Used to produce site-specifically phosphorylated proteins at a tyrosine residue via genetic code expansion versus other protein phosphorylation strategies.
RS Organism of Origin
Parent RS
RS Mutations
Y32S
L65A
F108K
Q109H
D158G
L162K
L65A
F108K
Q109H
D158G
L162K
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
C17A
U17aG
U20C
G37A
U47G
U17aG
U20C
G37A
U47G
RS/tRNA Availability
E. coli strain C321.ΔA.exp - Addgene: 49018
Used in what cell line?