RS/tRNA Foundational Publication Support
Neumann, Heinz, Sew Y Peak-Chew, and Jason W Chin. (2008) 2008. “Genetically Encoding N(Epsilon)-Acetyllysine In Recombinant Proteins.”. Nature Chemical Biology 4 (4): 232-4. doi:10.1038/nchembio.73.
RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
Nε-acetyl-L-lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
They are used for sites specifically studying post-translational modification acetylation events on particular lysine residues.
RS Organism of Origin
Parent RS
RS Mutations
D76G
L266V
L270I
Y271F
L274A
C313F
L266V
L270I
Y271F
L274A
C313F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
N/A
Multiple tRNAs?
N/A
RS/tRNA Availability
n/a
RS/tRNA Additional Notes
The D76G mutation was accidental and may not contribute to the AcK-encoding activity. Installed AcK (at 1 mM) into myoglobin(4) in E. coli with full fidelity and 1.5 mg/L yield. Also, enabled full-fidelity incorporation into superoxide dismutase(44). MbAcKRS1 has been superceded by the more efficient MbAckRS3, developed the following year by the same group.