RS/tRNA Foundational Publication Support
Neumann, Heinz, Sew Y Peak-Chew, and Jason W Chin. (2008) 2008. “Genetically Encoding N(Epsilon)-Acetyllysine In Recombinant Proteins.”. Nature Chemical Biology 4 (4): 232-4. doi:10.1038/nchembio.73.
RS/tRNA Usage Publications
Sun, Yanan, Yanchi Chen, Yaxin Xu, Yuqing Zhang, Minghao Lu, Manjia Li, Liyan Zhou, and Tao Peng. (jul) 2022. “Genetic Encoding Of Ε-N-L-Lactyllysine For Detecting Delactylase Activity In Living Cells.”. Chemical Communications (Cambridge, England) 58: 8544-8547. doi:10.1039/d2cc02643k.
RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
Nε-acetyl-L-lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
They are used for sites specifically studying post-translational modification acetylation events on particular lysine residues.
RS Organism of Origin
Parent RS
RS Mutations
L266V
L270I
Y271F
L274A
C313F
D76G
L270I
Y271F
L274A
C313F
D76G
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
N/A
Multiple tRNAs?
N/A
RS/tRNA Availability
It is not Commercially available but can be made by applying the mutation to Mb-PylRS/tRNA.
RS/tRNA Additional Notes
Installed AcK (at 1 mM) into myoglobin in E. coli with full fidelity and 1.5 mg/L yield. Also, enabled full-fidelity incorporation into superoxide dismutase. It has been superceded by AckRS3, developed the following year by the same group.