RS/tRNA Pair Development Year
2008
RS Organism of Origin
Parent RS
RS Mutations
L305I
Y306F
L309A
C348F
Y306F
L309A
C348F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
N/A
Multiple tRNAs?
N/A
RS/tRNA Availability
It is not Commercially available but can be made by applying the mutation to Mm-PylRS/tRNA.
Used in what cell line?
RS/tRNA Additional Notes
This RS is an AST of AcKRS1 from M.barkeri to M.mazei for use in mammalian cells (HEK293) and is shown to have moderately worse incorporation efficiency.