RS/tRNA Foundational Publication Support
Mukai, Takahito, Takatsugu Kobayashi, Nobumasa Hino, Tatsuo Yanagisawa, Kensaku Sakamoto, and Shigeyuki Yokoyama. (jul) 2008. “Adding L-Lysine Derivatives To The Genetic Code Of Mammalian Cells With Engineered Pyrrolysyl-Trna Synthetases”. Biochemical And Biophysical Research Communications 371: 818-822. doi:10.1016/j.bbrc.2008.04.164.
RS/tRNA Usage Publications
Roy, Gargi, Jason Reier, Andrew Garcia, Tom Martin, Megan Rice, Jihong Wang, Meagan Prophet, et al. (nov) 2019. “Development Of A High Yielding Expression Platform For The Introduction Of Non-Natural Amino Acids In Protein Sequences”. Mabs 12: 1684749. doi:10.1080/19420862.2019.1684749.
Jiang, Han-Kai, Man-Nee Lee, Jo-Chu Tsou, Kuan-Wen Chang, Hsueh-Wei Tseng, Kuang-Po Chen, Yaw-Kuen Li, and Yane-Shih Wang. (apr) 2020. “Linker And N-Terminal Domain Engineering Of Pyrrolysyl-Trna Synthetase For Substrate Range Shifting And Activity Enhancement”. Frontiers In Bioengineering And Biotechnology 8. doi:10.3389/fbioe.2020.00235.
RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
Nε-acetyl-L-lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
They are used for sites specifically studying post-translational modification acetylation events on particular lysine residues.
RS Organism of Origin
Parent RS
RS Mutations
L305I
Y306F
L309A
C348F
Y306F
L309A
C348F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
N/A
Multiple tRNAs?
N/A
RS/tRNA Availability
It is not Commercially available but can be made by applying the mutation to Mm-PylRS/tRNA.
Used in what cell line?
RS/tRNA Additional Notes
This RS is an AST of AcKRS1 from M.barkeri to M.mazei for use in mammalian cells (HEK293) and is shown to have moderately worse incorporation efficiency.