RS/tRNA Foundational Publication Support
Alfonta, Lital, Zhiwen Zhang, Sean Uryu, Joseph A Loo, and Peter G Schultz. (2003) 2003. “Site-Specific Incorporation Of A Redox-Active Amino Acid Into Proteins.”. Journal Of The American Chemical Society 125 (48): 14662-3.
RS/tRNA Usage Publications
Hayun, Hezi, Matt Coban, Ashok Kumar Bhagat, Eden Ozer, Lital Alfonta, Thomas R Caulfield, Evette S Radisky, and Niv Papo. (2023) 2023. “Utilizing Genetic Code Expansion To Modify N-Timp2 Specificity Towards Mmp-2, Mmp-9, And Mmp-14.”. Research Square. doi:10.21203/rs.3.rs-2446107/v1.
RS/tRNA Pair Development Year
2003
ncAA(s) Incorporated
3,4-Dihydroxy-L-phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Redox active amino acid
RS Organism of Origin
Parent RS
RS Mutations
Y32L
A67S
H70N
A167Q
A67S
H70N
A167Q
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
n/a
RS/tRNA Additional Notes
produced ~1 mg/L of myoglobin(4) compared to 5.4 mg/l for wt myoglobin. MS confirmed incorporation, but also showed a similar amount of a peak 16 Da lighter (which could represent incorporation of Tyr) and a lesser amount of a peak 51 Da lighter. Later papers using the RS showed high-fidelity incorporation into the proteins of interest.
used in 2020 paper "Genetically Expanded Reactive-Oxygen-Tolerant Alcohol Dehydrogenase II" and 2025 paper on "Enhanced Cell Adhesion Properties of a Collagen-Mimicking Peptide Through Site-Specific L-DOPA Incorporation" both not indexed in pubmed
used in 2020 paper "Genetically Expanded Reactive-Oxygen-Tolerant Alcohol Dehydrogenase II" and 2025 paper on "Enhanced Cell Adhesion Properties of a Collagen-Mimicking Peptide Through Site-Specific L-DOPA Incorporation" both not indexed in pubmed