RS/tRNA Foundational Publication Support
Sakamoto, Kensaku, Kazutaka Murayama, Kenji Oki, Fumie Iraha, Miyuki Kato-Murayama, Masahiro Takahashi, Kazumasa Ohtake, et al. (2009) 2009. “Genetic Encoding Of 3-Iodo-L-Tyrosine In Escherichia Coli For Single-Wavelength Anomalous Dispersion Phasing In Protein Crystallography.”. Structure (London, England : 1993) 17 (3): 335-44. doi:10.1016/j.str.2009.01.008.
RS/tRNA Protocols and Structural Information
Sakamoto, Kensaku, Kazutaka Murayama, Kenji Oki, Fumie Iraha, Miyuki Kato-Murayama, Masahiro Takahashi, Kazumasa Ohtake, et al. (2009) 2009. “Genetic Encoding Of 3-Iodo-L-Tyrosine In Escherichia Coli For Single-Wavelength Anomalous Dispersion Phasing In Protein Crystallography.”. Structure (London, England : 1993) 17 (3): 335-44. doi:10.1016/j.str.2009.01.008.
RS/tRNA Pair Development Year
2009
ncAA(s) Incorporated
3-iodo-L-tyrosine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
for anomalous dispersion phasing in protein crystallography.
RS Organism of Origin
Parent RS
RS Mutations
H70A
D158T
I159S
D286Y
D158T
I159S
D286Y
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
n/a
RS/tRNA Additional Notes
The D286Y mutation was added to improve recognition with the tRNA(CUA). The RS produced GST(25) (glutathione transferase) with high fidelity incorporation, and also ribosomal test protein with ncAA at multiple sites at ~5 mg/L. The foundational paper also reports a crystal structure of the RS in complex with 3-iodo-Tyr.