ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Yanagisawa, Tatsuo, Nobumasa Hino, Fumie Iraha, Takahito Mukai, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2012) 2012. “Wide-Range Protein Photo-Crosslinking Achieved By A Genetically Encoded N(Ε)-(Benzyloxycarbonyl)Lysine Derivative With A Diazirinyl Moiety.”. Molecular Biosystems 8 (4): 1131-5. doi:10.1039/c2mb05321g.
ncAA Usage Publications
Yanagisawa, Tatsuo, Nobumasa Hino, Fumie Iraha, Takahito Mukai, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2012) 2012. “Wide-Range Protein Photo-Crosslinking Achieved By A Genetically Encoded N(Ε)-(Benzyloxycarbonyl)Lysine Derivative With A Diazirinyl Moiety.”. Molecular Biosystems 8 (4): 1131-5. doi:10.1039/c2mb05321g.
ncAA Utility
Photo-cross-linking agent, but no tests were done on this ncAA in the founding paper. Crosslinking experiments were only done with TmdZLys
ncAA Source
Exogenous - Purchased
ncAA Availability
Can be purchased from https://www.benchchem.com/product/b555291
RS/tRNA Pair Usage Information
The Y306A,Y384F RS functioned better and is the RS of choice to use with this ncAA. In E coli DGH10B cells MS confirmed incorporation into GST residue 25 with yield of ~3 mg/L.
ncAA Synonyms
H-LYS(4-NITRO-Z)-OH
3557-90-2
(2S)-2-amino-6-[(4-nitrophenyl)methoxycarbonylamino]hexanoic Acid
N6-{[(4-nitrophenyl)methoxy]carbonyl}-L-lysine
(S)-2-Amino-6-((((4-nitrobenzyl)oxy)carbonyl)amino)hexanoic acid
L-Lys(4-NO2-Z)-OH
3557-90-2
(2S)-2-amino-6-[(4-nitrophenyl)methoxycarbonylamino]hexanoic Acid
N6-{[(4-nitrophenyl)methoxy]carbonyl}-L-lysine
(S)-2-Amino-6-((((4-nitrobenzyl)oxy)carbonyl)amino)hexanoic acid
L-Lys(4-NO2-Z)-OH
ChEBI ID
233096
PubChem Link