RS/tRNA Foundational Publication Support
Peng, Tao, and Howard C Hang. (2016) 2016. “Site-Specific Bioorthogonal Labeling For Fluorescence Imaging Of Intracellular Proteins In Living Cells.”. Journal Of The American Chemical Society 138 (43): 14423-14433.
RS/tRNA Usage Publications
Spence, Jennifer S, Ruina He, Hans-Heinrich Hoffmann, Tandrila Das, Emmanuelle Thinon, Charles M Rice, Tao Peng, Kartik Chandran, and Howard C Hang. (2019) 2019. “Ifitm3 Directly Engages And Shuttles Incoming Virus Particles To Lysosomes.”. Nature Chemical Biology 15 (3): 259-268. doi:10.1038/s41589-018-0213-2.
RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
TCO
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Reactive handle for IEDDA.
endo-BCN-L-Lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Reactive handle for SPAAC
exo-BCN-L-Lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Reactive handle for SPAAC
2'-aTCOK
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Biorthogonal ligation
RS Organism of Origin
Parent RS
RS Mutations
Y306A
Y384F
Y384F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
Can be purchased from Addgene Plasmid #122650
Used in what cell line?
RS/tRNA Additional Notes
Tested in live cell (HeLa):
Showed incorporation in HA-IFITM3-F8TAG at 50uM of exo-BCNK, endo-BCNK and 2'-aTOCK. Expression in presence of ncAA for 16 hours then depleted growth media for 6 hours after which they were labelled for 30 minutes with tetrazine flurophore. 2'-aTOCK was most efficiently incorportaed into all portien at higher protien expression levels compared to Exo-BCNK.
Expression of GFP-Y39TAG, showed incorporation for 2'a-TOCK was concentration independent.
Showed incorporation in HA-IFITM3-F8TAG at 50uM of exo-BCNK, endo-BCNK and 2'-aTOCK. Expression in presence of ncAA for 16 hours then depleted growth media for 6 hours after which they were labelled for 30 minutes with tetrazine flurophore. 2'-aTOCK was most efficiently incorportaed into all portien at higher protien expression levels compared to Exo-BCNK.
Expression of GFP-Y39TAG, showed incorporation for 2'a-TOCK was concentration independent.