RS/tRNA Foundational Publication Support
Mukai, Takahito, Takatsugu Kobayashi, Nobumasa Hino, Tatsuo Yanagisawa, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2008) 2008. “Adding L-Lysine Derivatives To The Genetic Code Of Mammalian Cells With Engineered Pyrrolysyl-Trna Synthetases.”. Biochemical And Biophysical Research Communications 371 (4): 818-22. doi:10.1016/j.bbrc.2008.04.164.
RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
Nε-acetyl-L-lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
They are used for sites specifically studying post-translational modification acetylation events on particular lysine residues.
RS Organism of Origin
Parent RS
RS Mutations
L305I
Y306F
L309A
C348F
Y306F
L309A
C348F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
The 4 mutations listed were incorporated from M. Barkeri AcLysRS binding pocket. Full length GRB2(Am-111)-FLAG was expressed in mammalian cells (HEK-293), which allowed for use of EBNA1-oriP system.