RS/tRNA Foundational Publication Support
Stokes, Audrey L, Shigeki J Miyake-Stoner, Jennifer C Peeler, Duy P Nguyen, Robert P Hammer, and Ryan A Mehl. (2009) 2009. “Enhancing The Utility Of Unnatural Amino Acid Synthetases By Manipulating Broad Substrate Specificity.”. Molecular Biosystems 5 (9): 1032-8. doi:10.1039/b904032c.
RS/tRNA Pair Development Year
2009
ncAA(s) Incorporated
3-Fluoro-4-nitro-p benzoyl-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
4-Nitro-p-benzoyl-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
RS Organism of Origin
Parent RS
RS Mutations
V164A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In DH10B cells, media contained 1mM 3-Fluoro-4-nitro-p benzoyl-L phenylalanine, under these conditions Bpa-RS-V164A produced approximately 600 mg/L sfGFP.
In DH10B cells, media contained1mM 4-Nitro-p-benzoyl-L phenylalanine, under these conditions Bpa-RS-V164A produced approximately 800 mg/L sfGFP.
Of note, Bpa-RS-V164A also had very general permissivity for other ncAAs, however it had the most yield of sfGFP when incorporating these two amino acids. Other synthetases created in the foundational study have their own entries containing the ncAAs they incorporated most effectively.
In DH10B cells, media contained1mM 4-Nitro-p-benzoyl-L phenylalanine, under these conditions Bpa-RS-V164A produced approximately 800 mg/L sfGFP.
Of note, Bpa-RS-V164A also had very general permissivity for other ncAAs, however it had the most yield of sfGFP when incorporating these two amino acids. Other synthetases created in the foundational study have their own entries containing the ncAAs they incorporated most effectively.