RS/tRNA Foundational Publication Support
Bryson, David I., Chenguang Fan, Li-Tao Guo, Corwin Miller, Dieter Söll, and David R. Liu. (dec) 2017. “Continuous Directed Evolution Of Aminoacyl-Trna Synthetases”. Nature Chemical Biology 13: 1253-1260. doi:10.1038/nchembio.2474.
RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
Nε-acetyl-L-lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
They are used for sites specifically studying post-translational modification acetylation events on particular lysine residues.
RS Organism of Origin
Parent RS
RS Mutations
V31I
T56P
H62Y
D76G
A100E
L266M
L270I
Y271F
L274A
C313F
T56P
H62Y
D76G
A100E
L266M
L270I
Y271F
L274A
C313F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
Addgene plasmid #104070
RS/tRNA Additional Notes
Created to test if adding the "IPYE" mutations to various PylRSs would improve their activity. In the presence of 1 mM AcK, adding the IPYE mutations to the MbAcK3RS (originally called MbAcKRS3) resulted in an ~10-fold increased efficiency of expression of a reporter sfGFP(2) (reaching ~17% efficiency). This efficiency was a bit lower than that achieved by the chAcK3RS(IPYE) (~22%) and a bit higher than that achieved by the MmAcK3RS(IPYE) (~12%).