RS/tRNA Foundational Publication Support
Serfling, Robert, Christian Lorenz, Maja Etzel, Gerda Schicht, Thore Böttke, Mario Mörl, and Irene Coin. (2018) 2018. “Designer Trnas For Efficient Incorporation Of Non-Canonical Amino Acids By The Pyrrolysine System In Mammalian Cells.”. Nucleic Acids Research 46 (1): 1-10. doi:10.1093/nar/gkx1156.
Nikić, Ivana, Gemma Estrada Girona, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Christine Koehler, Nataliia Shymanska V, Camilla Ventura Santos, Daniel Spitz, and Edward A Lemke. (2016) 2016. “Debugging Eukaryotic Genetic Code Expansion For Site-Specific Click-Paint Super-Resolution Microscopy.”. Angewandte Chemie (International Ed. In English) 55 (52): 16172-16176. doi:10.1002/anie.201608284.
RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
Boc-LysOH
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Hydroxy acid to introduce ester linkages into protein backbone
N6-(Benzyloxycarbonyl)-L-lysine (Z-Lys)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
RS Organism of Origin
Parent RS
RS Mutations
F271A
Y349F
Y349F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
U29aC mutation at the U29a:G41b pair
RS/tRNA Availability
Available on Addgene: https://www.addgene.org/105830/
Used in what cell line?
RS/tRNA Additional Notes
Tertiary Watson-Crick pair U19:A56 mutated to the more stable pair G19:C56 between D- and T-loop.
RS has an additional nuclear export signal (NES) for out competing any NLS import signal intrinsic to the PylRS.
Effective incorporation of Lys (boc) and zLys reported
RS has an additional nuclear export signal (NES) for out competing any NLS import signal intrinsic to the PylRS.
Effective incorporation of Lys (boc) and zLys reported