RS/tRNA Foundational Publication Support
Lee, Yan-Jiun, M Schmidt, Jeffery M Tharp, Annemarie Weber, Amber Koenig, Hong Zheng, Jianmin Gao, Marcey L Waters, Daniel Summerer, and Wenshe R Liu. (2016) 2016. “Genetically Encoded Fluorophenylalanines Enable Insights Into The Recognition Of Lysine Trimethylation By An Epigenetic Reader.”. Chemical Communications (Cambridge, England) 52 (85): 12606-12609. doi:10.1039/c6cc05959g.
RS/tRNA Pair Development Year
2017
RS Organism of Origin
Parent RS
RS Mutations
N346A
C348S
C348S
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
https://www.addgene.org/137908/
Used in what cell line?
RS/tRNA Additional Notes
Successfully incorporates fluorophenylalanines with fluorine substituents at the phenyl ring ranging from 2 to 5. Pentafluoro-phenylalanine(F5F) incorporation yielded 10 mg/L of full length sfGFP. Confirmed with ESI-MS
2,3,4,5-tetrafluorophenylalaine (F4F),14 3,4,5-trifluorophenylalanine (F3F), 3,5-difluorophenylalanine (F2F), and 3,4-difluorophenylalanine (F2F’) were also incorporated and yielded full length sfGFP. All were confirmed with ESI-MS, F2F' was shown to have displaced regular phenylalanine residues in sfGFP as well.
2,3,4,5-tetrafluorophenylalaine (F4F),14 3,4,5-trifluorophenylalanine (F3F), 3,5-difluorophenylalanine (F2F), and 3,4-difluorophenylalanine (F2F’) were also incorporated and yielded full length sfGFP. All were confirmed with ESI-MS, F2F' was shown to have displaced regular phenylalanine residues in sfGFP as well.