RS/tRNA Foundational Publication Support
Wang, Zhipeng A, Yadagiri Kurra, Xin Wang, Yu Zeng, Yan-Jiun Lee, Vangmayee Sharma, Hening Lin, Susie Dai, and Wenshe R Liu. (2017) 2017. “A Versatile Approach For Site-Specific Lysine Acylation In Proteins.”. Angewandte Chemie (International Ed. In English) 56 (6): 1643-1647. doi:10.1002/anie.201611415.
RS/tRNA Pair Development Year
2018
ncAA(s) Incorporated
Azidonorleucine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Biorthogonal Ligation, Click Chemistry labeling with fluorescence or affinity labels. Specifically used for cell-specific proteomics studies using "bioorthogonal noncanonical amino acid tagging" (BONCAT) proteomics. Contrasts with Azidohomoalanine which does not allow cell-specific studies.
RS Organism of Origin
Parent RS
RS Mutations
Y306L
C348I
Y384F
C348I
Y384F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
https://www.addgene.org/172482/
Used in what cell line?
RS/tRNA Additional Notes
Expressions of full length sfGFP protein was achieved with AznLRS and 5mM Azidonorleucine, with a yield of 20 mg/L. Results were confirmed with ESI-MS.
A ubiquitin variant Ub-K48AznL with a C-terminal 6×His tag and AznL incorporated at the original K48 position was produced at 10 mg/L under similar conditions. MALDI-TOF MS confirmed their results.
A ubiquitin variant Ub-K48AznL with a C-terminal 6×His tag and AznL incorporated at the original K48 position was produced at 10 mg/L under similar conditions. MALDI-TOF MS confirmed their results.