ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Zhu, Phillip, Philip R Gafken, Ryan A Mehl, and Richard B Cooley. (2019) 2019. “A Highly Versatile Expression System For The Production Of Multiply Phosphorylated Proteins.”. Acs Chemical Biology 14 (7): 1564-1572. doi:10.1021/acschembio.9b00307.
ncAA Protocols
Zhu, Phillip, Ryan A Mehl, and Richard B Cooley. (2022) 2022. “Site-Specific Incorporation Of Phosphoserine Into Recombinant Proteins In .”. Bio-Protocol 12 (21). doi:10.21769/BioProtoc.4541.
ncAA Usage Publications
Stuber, Katrin, Tobias Schneider, Jill Werner, Michael Kovermann, Andreas Marx, and Martin Scheffner. (2021) 2021. “Structural And Functional Consequences Of Nedd8 Phosphorylation.”. Nature Communications 12 (1): 5939. doi:10.1038/s41467-021-26189-9.
Vesely, Cat Hoang, Patrick N Reardon, Zhen Yu, Elisar Barbar, Ryan A Mehl, and Richard B Cooley. (2022) 2022. “Accessing Isotopically Labeled Proteins Containing Genetically Encoded Phosphoserine For Nmr With Optimized Expression Conditions.”. The Journal Of Biological Chemistry 298 (12): 102613. doi:10.1016/j.jbc.2022.102613.
ncAA Utility
Used to produce proteins site-specifically phosphorylated at serine residues, typically at positions where phosphoserine naturally occurs as a post-translational modification
ncAA Source
Endogenous - Biosynthesized
ncAA Availability
pSer is the natural precursor to Serine, and by knocking out the SerB enzyme (catalyzing last step in Ser production pathway), intracellular pSer builds up and can be used for GCE. The relevant RS/tRNA database entries give information on the ΔserB cell lines used.
ncAA Synonyms
(2S)-2-amino-3-(phosphonooxy)propanoic acid
O-phosphoserine
pSer
O-phospho-L-serine
O-phosphono-L-serine
O-phosphoserine
pSer
O-phospho-L-serine
O-phosphono-L-serine
ChEBI ID
15811
PubChem Link
PDB Code
SEP