GCE Publications

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2023 An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids. Ke Liu, Ling Jiang, Shuang Ma, Zhongdi Song, Lun Wang, Qunfeng Zhang, Renhao Xu, Lirong Yang, Jianping Wu, Haoran Yu
2023 Non-canonical amino acid incorporation into AAV5 capsid enhances lung transduction in mice. Hao Chang, Ailing Du, Jun Jiang, Lingzhi Ren, Nan Liu, Xuntao Zhou, Jialing Liang, Guangping Gao, Dan Wang
2023 Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation. Chasity Janosko, Olivia Shade, Taylor Courtney, Trevor Horst, Melinda Liu, Sagar Khare, Alexander Deiters
2023 Selective Inhibition of Kinase Activity in Mammalian Cells by Bioorthogonal Ligand Tethering. Jinghao Chen, Yang Huang, Wen-Biao Gan, Yu-Hsuan Tsai
2023 Shedding light on ORAI1 channel with genetic code expansion. Sher Ali, Guolin Ma, Yubin Zhou
2023 Residue selective crosslinking of proteins through photoactivatable or proximity-enabled reactivity. Jun Liu, Bing Yang, Lei Wang
2023 Genetic encoding of 3-nitro-tyrosine reveals the impacts of 14-3-3 nitration on client binding and dephosphorylation. Phillip Zhu, Kyle Nguyen, Aidan Estelle, Nikolai Sluchanko, Ryan Mehl, Richard Cooley
2023 Site-Specific Introduction of Bioorthogonal Handles to Nanopores by Genetic Code Expansion. Jing Yang, Kefan Wang, Shanyu Zhang, Xiaocui Zheng, Tongxiao Cui, Xian Yang, Yao Liu, Di Lu, Yuqin Wang, Xiao Tian, Panke Zhang, Shuo Huang, Ran Xie
2022 Enhanced access to the human phosphoproteome with genetically encoded phosphothreonine. Jack Moen, Kyle Mohler, Svetlana Rogulina, Xiaojian Shi, Hongying Shen, Jesse Rinehart
2022 Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation. Yuda Chen, Shikai Jin, Mengxi Zhang, Yu Hu, Kuan-Lin Wu, Anna Chung, Shichao Wang, Zeru Tian, Yixian Wang, Peter Wolynes, Han Xiao
2022 Expanding the eukaryotic genetic code with a biosynthesized 21st amino acid. Kuan-Lin Wu, Joshua Moore, Mitchell Miller, Yuda Chen, Catherine Lee, Weijun Xu, Zane Peng, Qinghui Duan, George Phillips, Rosa Uribe, Han Xiao
2022 Enhanced incorporation of subnanometer tags into cellular proteins for fluorescence nanoscopy via optimized genetic code expansion. Tiberiu Mihaila, Carina Bäte, Lynn Ostersehlt, Jasmin Pape, Jan Keller-Findeisen, Steffen Sahl, Stefan Hell
2022 Nanobody assemblies with fully flexible topology enabled by genetically encoded tetrazine amino acids. Elise Van Fossen, Riley Bednar, Subhashis Jana, Rachel Franklin, Joseph Beckman, Andrew Karplus, Ryan Mehl
2022 Measuring the tolerance of the genetic code to altered codon size. Erika DeBenedictis, Dieter Söll, Kevin Esvelt
2022 Making Sense of "Nonsense" and More: Challenges and Opportunities in the Genetic Code Expansion, in the World of tRNA Modifications. Olubodun Lateef, Michael Akintubosun, Olamide Olaoba, Sunday Samson, Malgorzata Adamczyk
2022 Recent Technologies for Genetic Code Expansion and their Implications on Synthetic Biology Applications. Hongting Tang, Pan Zhang, Xiaozhou Luo
2022 Engineering Translation Components for Genetic Code Expansion. Sooin Kim, Hanbin Yi, Yurie Kim, Hyun Lee
2022 Exploration of Pyrrolysyl-tRNA Synthetase Activity in Yeast. Jessica Stieglitz, Priyanka Lahiri, Matthew Stout, James Van Deventer
2022 TDP-43 safeguards the embryo genome from L1 retrotransposition. Ten Li, Kensaku Murano, Tomohiro Kitano, Youjia Guo, Lumi Negishi, Haruhiko Siomi
2022 Photoswitching of Feedback Inhibition by Tryptophan in Anthranilate Synthase. Ashok Bhagat, Sandra Schlee, Kristina Straub, Julian Nazet, Patricia Luckner, Astrid Bruckmann, Reinhard Sterner
2022 FRET Monitoring of a Nonribosomal Peptide Synthetase Elongation Module Reveals Carrier Protein Shuttling between Catalytic Domains. Jennifer Rüschenbaum, Wieland Steinchen, Florian Mayerthaler, Anna-Lena Feldberg, Henning Mootz
2022 Site-Specific Protein Labeling and Generation of Defined Ubiquitin-Protein Conjugates Using an Asparaginyl Endopeptidase. Maximilian Fottner, Johannes Heimgärtner, Maximilian Gantz, Rahel Mühlhofer, Timon Nast-Kolb, Kathrin Lang
2022 A Chemical Biology Primer for NMR Spectroscopists. Evan Clark, Elanor Sievers, Galia Debelouchina
2022 Spontaneous Orthogonal Protein Crosslinking via a Genetically Encoded 2-Carboxy-4-Aryl-1,2,3-Triazole. Yali Xu, Abdur Rahim, Qing Lin
2022 The Pyrrolysyl-tRNA Synthetase Activity can be Improved by a P188 Mutation that Stabilizes the Full-Length Enzyme. Chia-Chuan Cho, Lauren Blankenship, Xinyu Ma, Shiqing Xu, Wenshe Liu