ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Sakamoto, Kensaku, Kazutaka Murayama, Kenji Oki, Fumie Iraha, Miyuki Kato-Murayama, Masahiro Takahashi, Kazumasa Ohtake, et al. (2009) 2009. “Genetic Encoding Of 3-Iodo-L-Tyrosine In Escherichia Coli For Single-Wavelength Anomalous Dispersion Phasing In Protein Crystallography.”. Structure (London, England : 1993) 17 (3): 335-44. doi:10.1016/j.str.2009.01.008.
Iraha, Fumie, Kenji Oki, Takatsugu Kobayashi, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Shigeyuki Yokoyama, and Kensaku Sakamoto. (2010) 2010. “Functional Replacement Of The Endogenous Tyrosyl-Trna Synthetase-Trnatyr Pair By The Archaeal Tyrosine Pair In Escherichia Coli For Genetic Code Expansion.”. Nucleic Acids Research 38 (11): 3682-91. doi:10.1093/nar/gkq080.
ncAA Protocols
Hino, Nobumasa, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2012) 2012. “Site-Specific Incorporation Of Unnatural Amino Acids Into Proteins In Mammalian Cells.”. Methods In Molecular Biology (Clifton, N.j.) 794: 215-28. doi:10.1007/978-1-61779-331-8_13.
ncAA Utility
for anomalous dispersion phasing in protein crystallography.
ncAA Source
Exogenous - Synthesized
ncAA Availability
Sigma-Aldrich catalog no. I8250
RS/tRNA Pair Usage Information
In E coli expressions, the Mj RS leads to higher protein expression than does the Ec RS (in the 2010 foundational paper), and is also easier to use as it does not require a special E. coli strain. The Ec RS has been used in CHO and HEK293 cells.
ChEBI ID
27847
PubChem Link
PDB Code
IYR