RS/tRNA Foundational Publication Support
Furman, Jennifer L, Mingchao Kang, Seihyun Choi, Yu Cao, Erik D Wold, Sophie B Sun, Vaughn Smider V, Peter G Schultz, and Chan Hyuk Kim. (2014) 2014. “A Genetically Encoded Aza-Michael Acceptor For Covalent Cross-Linking Of Protein-Receptor Complexes.”. Journal Of The American Chemical Society 136 (23): 8411-7. doi:10.1021/ja502851h.
RS/tRNA Pair Development Year
2014
ncAA(s) Incorporated
p-Acrylamido-(S)-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Reactive handle for Michael additions, cross-linker
RS Organism of Origin
Parent RS
RS Mutations
T32V
L65T
F108H
Q109G
D158G
L162E
D286R
L65T
F108H
Q109G
D158G
L162E
D286R
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In BL21(DE3) cells, fluorescence assay was performed using GFP-AcrF151 with the addition of 1mM p-Acrylamido-(S)-L phenylalanine yielding a 2-fold increase in GFP expression versus the absence of p-Acrylamido-(S)-L phenylalanine. Characterization using SDS-PAGE and ESI-MS was performed with the observed mass not precisely correlating to the literature mass, but was explained as potentially corresponding to a GSH adduct of GFP-AcrF151.