RS/tRNA Foundational Publication Support
Nguyen, Duy P, Thomas Elliott, Matthew Holt, Tom W Muir, and Jason W Chin. (2011) 2011. “Genetically Encoded 1,2-Aminothiols Facilitate Rapid And Site-Specific Protein Labeling Via A Bio-Orthogonal Cyanobenzothiazole Condensation.”. Journal Of The American Chemical Society 133 (30): 11418-21. doi:10.1021/ja203111c.
RS/tRNA Pair Development Year
2011
ncAA(s) Incorporated
l-Cys-e-Lys
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Condenses with 2-cyanobenzothiazole for protein modification.
D-Cys-ε-Lys (“(S,S)”)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Reactive handle for NCL
RS Organism of Origin
Parent RS
RS Mutations
C313V
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
CysKRS incorporated 10mm l-Cys-e-Lys, and 10mm D-Cys-ε-Lys 9-fold and 6-fold more efficiently than wt-mbPylRS respectively using GFP assays and immunoblotting using myoglobin-His6.
D-Cys-ε-Lys was incorporated into a T4 lysozyme using CysKRS, which was confirmed by ESI-MS.
D-Cys-ε-Lys was incorporated into a T4 lysozyme using CysKRS, which was confirmed by ESI-MS.