RS/tRNA Foundational Publication Support
Lemke, Edward A, Daniel Summerer, Bernhard H Geierstanger, Scott M Brittain, and Peter G Schultz. (2007) 2007. “Control Of Protein Phosphorylation With A Genetically Encoded Photocaged Amino Acid.”. Nature Chemical Biology 3 (12): 769-72.
RS/tRNA Pair Development Year
2007
ncAA(s) Incorporated
4,5-Dimethoxy-2 nitrobenzylserine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Photocaged serine
RS Organism of Origin
Parent RS
RS Mutations
M40G
L41Q
Y499L
Y527G
H537F
T252A
L41Q
Y499L
Y527G
H537F
T252A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In SCY4 yeast cells, hSOD-33TAG-His6 was co-transformed alongside LeuRS-BH5-T252A in the presence and absence of 1mM 4,5-Dimethoxy-2 nitrobenzylserine, yielding 0.8mg/L mutant hSOD protein with ncAA and none without. Gel electrophoresis and ESI-MS showed high fidelity and confirmed incorporation of 4,5-Dimethoxy-2 nitrobenzylserine.
LeuRS-BH5-T252A was also shown to be effective for the expression of Pho4-GFP fusion constructs in the presence of 4,5-Dimethoxy-2 nitrobenzylserine.
LeuRS-BH5-T252A was also shown to be effective for the expression of Pho4-GFP fusion constructs in the presence of 4,5-Dimethoxy-2 nitrobenzylserine.