RS/tRNA Foundational Publication Support
Summerer, Daniel, Shuo Chen, Ning Wu, Alexander Deiters, Jason W Chin, and Peter G Schultz. (2006) 2006. “A Genetically Encoded Fluorescent Amino Acid.”. Proceedings Of The National Academy Of Sciences Of The United States Of America 103 (26): 9785-9.
RS/tRNA Pair Development Year
2006
ncAA(s) Incorporated
DansylAla
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Fluorescent amino acid
RS Organism of Origin
Parent RS
RS Mutations
M40A
L41N
Y499I
Y527G
H537T
T252A
L41N
Y499I
Y527G
H537T
T252A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
Using PGK1 promoter, hSOD harboring Dansylalanine was expressed at 1.11mg/L at position 33.
High fidelity for the incorporation of Dansylalanine by LeuRSB8T252A was confirmed by MALDI-TOF mass spec with an observed m/z of 16,802.
High fidelity for the incorporation of Dansylalanine by LeuRSB8T252A was confirmed by MALDI-TOF mass spec with an observed m/z of 16,802.