RS/tRNA Foundational Publication Support
Summerer, Daniel, Shuo Chen, Ning Wu, Alexander Deiters, Jason W Chin, and Peter G Schultz. (2006) 2006. “A Genetically Encoded Fluorescent Amino Acid.”. Proceedings Of The National Academy Of Sciences Of The United States Of America 103 (26): 9785-9.
RS/tRNA Usage Publications
Amadei, Matteo, Antonella Niro, Maria Rosaria Fullone, Rossella Miele, Fabio Polticelli, Giovanni Musci, and Maria Carmela Bonaccorsi di Patti. (2023) 2023. “Genetic Incorporation Of Dansylalanine In Human Ferroportin To Probe The Alternating Access Mechanism Of Iron Transport.”. International Journal Of Molecular Sciences 24 (15). doi:10.3390/ijms241511919.
RS/tRNA Pair Development Year
2006
ncAA(s) Incorporated
DansylAla
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Fluorescent amino acid
RS Organism of Origin
Parent RS
RS Mutations
M40A
L41N
Y499I
Y527G
H537T
T252A
L41N
Y499I
Y527G
H537T
T252A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
The T252A mutation, shown earlier to cause "correctly" charged Leu-tRNALeu to be hydrolyzed, was added to lower the RS-directed incorporation of Leu instead of the ncAA. This RS, in the presence of dansylalanine, yielded hSOD(33) at ~1 mg/L with high fidelity confirmed by MS.
2023 application paper used the RS in P. pastoris
2023 application paper used the RS in P. pastoris