RS/tRNA Foundational Publication Support
Chen, Shuo, and Meng-Lin Tsao. (2013) 2013. “Genetic Incorporation Of A 2-Naphthol Group Into Proteins For Site-Specific Azo Coupling.”. Bioconjugate Chemistry 24 (10): 1645-9. doi:10.1021/bc400168u.
RS/tRNA Pair Development Year
2013
ncAA(s) Incorporated
2-amino-3-(6-hydroxy 2-naphthyl)-propanoic acid
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Reactive handle for azo coupling
RS Organism of Origin
Parent RS
RS Mutations
Y32E
L65T
D158S
I159A
H160P
Y161T
L162Q
A167W
D286R
L65T
D158S
I159A
H160P
Y161T
L162Q
A167W
D286R
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
NpOH-RS1 incorporated 2-amino-3-(6-hydroxy 2-naphthyl)-propanoic acid(NpOH) into target protein with high fidelity via substitution of the Lys7 site, yielding 7mg/L of Z-domain mutant. These results were confirmed by ESI-MS and SDS-page results.
Another RS mutant was acquired which yielded similar results, however it was not reported to do anything substantially different from NpOH-RS1, and is mentioned here instead of receiving its own page. The mutant NpOH-RS2 had the mutations Y32E, L65 V, K90E, I159A, H160W, Y161G, L162Q, A167I, and D286R.
Another RS mutant was acquired which yielded similar results, however it was not reported to do anything substantially different from NpOH-RS1, and is mentioned here instead of receiving its own page. The mutant NpOH-RS2 had the mutations Y32E, L65 V, K90E, I159A, H160W, Y161G, L162Q, A167I, and D286R.