RS/tRNA Foundational Publication Support
Guo, Jiantao, Jiangyun Wang, Christopher Anderson, and Peter G Schultz. (2008) 2008. “Addition Of An Alpha-Hydroxy Acid To The Genetic Code Of Bacteria.”. Angewandte Chemie (International Ed. In English) 47 (4): 722-5.
RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
p-Hydroxy-phenyllactic acid
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Hydroxy acid to introduce ester linkages into protein backbone
RS Organism of Origin
Parent RS
RS Mutations
Gln155Arg
Gln173Gly
Ile176Val
Gln173Gly
Ile176Val
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In DH10B cells, mutated sperm whale myoglobin with a His6 tag was co-transformed with PlaRS/MjtRNAtyr in the presence or absence of 1mM of p-Hydroxy-phenyllactic acid. 2-3mg/L of full yield mutant myoglobin was only found in the presence of ncAA. Results confirmed by ESI-MS