RS/tRNA Foundational Publication Support
Park, Hee-Sung, Michael J Hohn, Takuya Umehara, Li-Tao Guo, Edith M Osborne, Jack Benner, Christopher J Noren, Jesse Rinehart, and Dieter Söll. (2011) 2011. “Expanding The Genetic Code Of Escherichia Coli With Phosphoserine.”. Science (New York, N.y.) 333 (6046): 1151-4. doi:10.1126/science.1207203.
Heinemann, Ilka U, Alexis J Rovner, Hans R Aerni, Svetlana Rogulina, Laura Cheng, William Olds, Jonathan T Fischer, Dieter Söll, Farren J Isaacs, and Jesse Rinehart. (2012) 2012. “Enhanced Phosphoserine Insertion During Escherichia Coli Protein Synthesis Via Partial Uag Codon Reassignment And Release Factor 1 Deletion.”. Febs Letters 586 (20): 3716-22. doi:10.1016/j.febslet.2012.08.031.
Oza, Javin P, Hans R Aerni, Natasha L Pirman, Karl Barber, Charlotte Haar, Svetlana Rogulina, Matthew Amrofell, Farren J Isaacs, Jesse Rinehart, and Michael C Jewett. (2015) 2015. “Robust Production Of Recombinant Phosphoproteins Using Cell-Free Protein Synthesis.”. Nature Communications 6: 8168. doi:10.1038/ncomms9168.
RS/tRNA Usage Publications
Aerni, Hans R, Mark A Shifman, Svetlana Rogulina, Patrick O'Donoghue, and Jesse Rinehart. (2015) 2015. “Revealing The Amino Acid Composition Of Proteins Within An Expanded Genetic Code.”. Nucleic Acids Research 43 (2): e8. doi:10.1093/nar/gku1087.
Sawyer, Nicholas, Brandon M Gassaway, Adrian D Haimovich, Farren J Isaacs, Jesse Rinehart, and Lynne Regan. (2014) 2014. “Designed Phosphoprotein Recognition In Escherichia Coli.”. Acs Chemical Biology 9 (11): 2502-7. doi:10.1021/cb500658w.
Liu, Dong, Yingying Liu, Hua-Zhen Duan, Xinjie Chen, Yanan Wang, Ting Wang, Qing Yu, Yong-Xiang Chen, and Yuan Lu. (2023) 2023. “Customized Synthesis Of Phosphoprotein Bearing Phosphoserine Or Its Nonhydrolyzable Analog.”. Synthetic And Systems Biotechnology 8 (1): 69-78. doi:10.1016/j.synbio.2022.11.004.
RS/tRNA Pair Development Year
2011
ncAA(s) Incorporated
phosphoserine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Used to produce proteins site-specifically phosphorylated at serine residues, typically at positions where phosphoserine naturally occurs as a post-translational modification
RS Organism of Origin
Parent RS
RS Mutations
none
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
C20U
RS/tRNA Availability
Addgene #34623 has the SepRS and EFSep, and Addgene #34626 has the Sep-tRNA and protein-of-interest genes
Used in what cell line?
RS/tRNA Additional Notes
This system does not achieve high fidelity pSer incorporation and has been superseded by GCE systems using SepRS2 (for protein production), and using SepRS9 (for studies of the human phosphoproteome expresssed in E. coli).