RS/tRNA Foundational Publication Support
Zeng, Huaqiang, Jianming Xie, and Peter G Schultz. (2006) 2006. “Genetic Introduction Of A Diketone-Containing Amino Acid Into Proteins.”. Bioorganic & Medicinal Chemistry Letters 16 (20): 5356-9.
RS/tRNA Pair Development Year
2006
ncAA(s) Incorporated
tert-butyloxycarbonyl
ncAA Structure (png, jpg, jpeg)
![(2S)-2-amino-3-[4-(3-oxobutanoyl)phenyl]propanoic acid](/sites/gce4all/files/styles/medium/public/2024-12/Page_3_Structure_4.png?itok=ExG3c9Xv)
ncAA Utility
Labelling with hydroxylamines
RS Organism of Origin
Parent RS
RS Mutations
Y32G
D158G
F65V
F108T
L162S
D158G
F65V
F108T
L162S
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In E. coli, t-BocRS was used in the presence or absence of 1mM tert-butyloxycarbonyl to express Z-domain protein containing a His6 tag in minimal media. Expression was induced by IPTG resulting in 1.6mg/L yield of mutant protein only in the presence of 1mM tert-butyloxycarbonyl.