RS/tRNA Foundational Publication Support
Seitchik, Jason L, Jennifer C Peeler, Michael T Taylor, Melissa L Blackman, Timothy W Rhoads, Richard B Cooley, Christian Refakis, Joseph M Fox, and Ryan A Mehl. (2012) 2012. “Genetically Encoded Tetrazine Amino Acid Directs Rapid Site-Specific In Vivo Bioorthogonal Ligation With Trans-Cyclooctenes.”. Journal Of The American Chemical Society 134 (6): 2898-901. doi:10.1021/ja2109745.
RS/tRNA Pair Development Year
2013
ncAA(s) Incorporated
Tet-v1.0 (4-(6-methyl-s-tetrazin 3-yl)amino-L phenylalanine)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Reactive handle for tetrazine ligation
RS Organism of Origin
Parent RS
RS Mutations
Y32E
L65A
A107E
F108P
Q109S
D158G
L162G
L65A
A107E
F108P
Q109S
D158G
L162G
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In DH10B cells, MjYRS/tRNAtyr was cloned onto a pDule vector, then co-transformed with pBad-sfGFP-150TAG in the presence or absence of 1mM 4-(6-methyl-s-tetrazin 3-yl)amino-L phenylalanine. Only in the presence of ncAA was full-length sGFP observed. Results were confirmed by ESI-MS and SDS-PAGE.