RS/tRNA Foundational Publication Support
Blizzard, Robert J, Dakota R Backus, Wes Brown, Christopher G Bazewicz, Yi Li, and Ryan A Mehl. (2015) 2015. “Ideal Bioorthogonal Reactions Using A Site-Specifically Encoded Tetrazine Amino Acid”. American Chemical Society. doi: 10.1021/jacs.5b03275 .
RS/tRNA Pair Development Year
2015
RS Organism of Origin
Parent RS
RS Mutations
T32G
L65Q
F108S
Q109D
D158S
L162N
L65Q
F108S
Q109D
D158S
L162N
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In DH10B cells, Tet-v2.0RS was cloned onto a pDule vector then co-transformed with pBad GFP-TAG150 in the presence or absence of 1mM Tet-v2.0, yielding 13mg/L of GFP after purification. Results were confirmed with ESI-Q mass analysis.