RS/tRNA Foundational Publication Support
Jackson, Jennifer C, Jared T Hammill, and Ryan A Mehl. (2007) 2007. “Site-Specific Incorporation Of A (19)F-Amino Acid Into Proteins As An Nmr Probe For Characterizing Protein Structure And Reactivity.”. Journal Of The American Chemical Society 129 (5): 1160-6.
RS/tRNA Usage Publications
Jackson, Jennifer C, Sean P Duffy, Kenneth R Hess, and Ryan A Mehl. (2006) 2006. “Improving Nature's Enzyme Active Site With Genetically Encoded Unnatural Amino Acids.”. Journal Of The American Chemical Society 128 (34): 11124-7.
RS/tRNA Pair Development Year
2007
ncAA(s) Incorporated
Trifluoromethyl-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
19F Probe for protein
NMR
NMR
p-Methyl-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Improving enzyme activity of a nitroreductase
RS Organism of Origin
Parent RS
RS Mutations
Y32L
L65A
F108S
H109H
D158A
L162M
L65A
F108S
H109H
D158A
L162M
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In DH10B cells, pDule-tfm-Phe1 plasmid bearing tfm-PheRS was co-transformed with pTrc-NTR-124TAG in minimal media, in the presence or absence of 1mM trifluoromethyl-L phenylalanine(tfm-Phe). Full-length protein was produced in the presence of 1mM tfm-Phe only, with yields varying from 12-24mg/L at site 124, and yields varying from 10-16mg/L at site 36.
When incorporating p-Methyl L Phenylalanine targeting site 124, high efficiency and fidelity was observed.
When incorporating p-Methyl L Phenylalanine targeting site 124, high efficiency and fidelity was observed.