RS/tRNA Foundational Publication Support
Nguyen, Duy P, Thomas Elliott, Matthew Holt, Tom W Muir, and Jason W Chin. (2011) 2011. “Genetically Encoded 1,2-Aminothiols Facilitate Rapid And Site-Specific Protein Labeling Via A Bio-Orthogonal Cyanobenzothiazole Condensation.”. Journal Of The American Chemical Society 133 (30): 11418-21. doi:10.1021/ja203111c.
RS/tRNA Pair Development Year
2011
ncAA(s) Incorporated
Ne-L-Thiapropyl-L-lysine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Precursor to NCL reactive handle
RS Organism of Origin
Parent RS
RS Mutations
A267S
C313V
M315F
D344G
C313V
M315F
D344G
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
ThzKRS incorporated 10mm Ne-L-Thiapropyl-L-lysine, resulting in 16-fold more GFP production than MbPylRS/tRNA, shown by GFP assays and western blotting.
In T4 Lysozyme, Ne-L-Thiapropyl-L-lysine was incorporated at position 83 at 2 mg/L in the presence of ThzKRS, confirmed by ESI-MS. This system was then used to test the effectiveness of CBT-fluorescein site-specific labeling, and dual-labeling by using calmodulin.
In T4 Lysozyme, Ne-L-Thiapropyl-L-lysine was incorporated at position 83 at 2 mg/L in the presence of ThzKRS, confirmed by ESI-MS. This system was then used to test the effectiveness of CBT-fluorescein site-specific labeling, and dual-labeling by using calmodulin.