RS/tRNA Foundational Publication Support
Bose, Mohua, Dan Groff, Jianming Xie, Eric Brustad, and Peter G Schultz. (2006) 2006. “The Incorporation Of A Photoisomerizable Amino Acid Into Proteins In E. Coli.”. Journal Of The American Chemical Society 128 (2): 388-9.
RS/tRNA Pair Development Year
2005
ncAA(s) Incorporated
Phe-4’-azobenzene
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Photo-isomerizable, used to block binding pockets
RS Organism of Origin
Parent RS
RS Mutations
Tyr32Gly
Leu65Glu
Phe108Ala
Gln109Glu
Asp158Gly
Leu162His
Leu65Glu
Phe108Ala
Gln109Glu
Asp158Gly
Leu162His
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In E. coli cells, AzoPheRS/MjtRNAtyr was used in media supplemented with and without 1mM Phe-4’-azobenzene(AzoPhe) to express sperm whale myoglobin containing a C-terminal His6 tag. Mutated myoglobin was only observed in the presence of AzoPhe, with results confirmed by ESI-MS.
Additionally, the AzoPheRS/MjtRNAtyr was used to express mutated and wild-type catabolite activator protein in E. coli cells. When performed in the presence of 1mM AzoPhe, 1.5mg/L mutant and 3mg/L wild-type protein were acquired.
Additionally, the AzoPheRS/MjtRNAtyr was used to express mutated and wild-type catabolite activator protein in E. coli cells. When performed in the presence of 1mM AzoPhe, 1.5mg/L mutant and 3mg/L wild-type protein were acquired.