RS/tRNA Foundational Publication Support
Brustad, Eric, Mark L Bushey, Jae Wook Lee, Dan Groff, Wenshe Liu, and Peter G Schultz. (2008) 2008. “A Genetically Encoded Boronate-Containing Amino Acid.”. Angewandte Chemie (International Ed. In English) 47 (43): 8220-3. doi:10.1002/anie.200803240.
RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
p-boronophenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
enabled one-step "scarless" protein purification procedure, and can be used to site-specifically label proteins using various boronic acid chemistries. Also, in second foundational paper was used in creating a fluorescent probe for peroxinitrite.
RS Organism of Origin
Parent RS
RS Mutations
Y32S
L65A
H70M
D158S
L162E
L65A
H70M
D158S
L162E
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
C17A
U17aG
U20C
G37A
U47G
U17aG
U20C
G37A
U47G
RS/tRNA Availability
n/a
Used in what cell line?
RS/tRNA Additional Notes
using 1 mM ncAA yielded 15 mg/L Z-domain(7) with high fidelity confirmed by MS. Also incorporated into T4 lysozyme(82).
Two additional RSs were selected but not fully characterized. One differed by having H70 unchanged, the other differed by having H70 unchanged, Y32G and D158A.
Two additional RSs were selected but not fully characterized. One differed by having H70 unchanged, the other differed by having H70 unchanged, Y32G and D158A.