RS/tRNA Foundational Publication Support
Stokes, Audrey L, Shigeki J Miyake-Stoner, Jennifer C Peeler, Duy P Nguyen, Robert P Hammer, and Ryan A Mehl. (2009) 2009. “Enhancing The Utility Of Unnatural Amino Acid Synthetases By Manipulating Broad Substrate Specificity.”. Molecular Biosystems 5 (9): 1032-8. doi:10.1039/b904032c.
RS/tRNA Pair Development Year
2009
ncAA(s) Incorporated
3-Fluoro-4-nitro-p benzoyl-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
4-Nitro-p-benzoyl-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
RS Organism of Origin
Parent RS
RS Mutations
Y32G
E107P
D158T
I159S
V164A
E107P
D158T
I159S
V164A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
C17A
U17aG
U20C
G37A
U47G
U17aG
U20C
G37A
U47G
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
DH10B cells with 1 mM 3F,4-nitro-Bpa yielded ~600 mg/L sfGFP(150), and with 1 mM 4-nitro-Bpa produced ~800 mg/L sfGFP(150) compared with 1800 mg/L for WT sfGFP. Also worked well with Bpa (~600 mg/L) and 4F-Bpa (~850 mg/L) but another RS from the study incorporated those ncAAs more efficiently.
This paper is also the one originating the use of sfGFP150 as a standard expression probe.
This paper is also the one originating the use of sfGFP150 as a standard expression probe.