CysKRS

RS/tRNA Foundational Publication Support

Nguyen, Duy P, Thomas Elliott, Matthew Holt, Tom W Muir, and Jason W Chin. (2011) 2011. “Genetically Encoded 1,2-Aminothiols Facilitate Rapid And Site-Specific Protein Labeling Via A Bio-Orthogonal Cyanobenzothiazole Condensation.”. Journal Of The American Chemical Society 133 (30): 11418-21. doi:10.1021/ja203111c.

RS/tRNA Pair Development Year
2011

ncAA(s) Incorporated

l-Cys-e-Lys

ncAA Structure (png, jpg, jpeg)
N6-L -Cysteinyl-L-lysine
ncAA Utility
Condenses with 2-cyanobenzothiazole for protein modification.


RS Organism of Origin
Parent RS
RS Mutations
C313V

tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA

RS/tRNA Availability
N/A
Used in what cell line?

RS/tRNA Additional Notes
CysKRS incorporated 10mm l-Cys-e-Lys, and 10mm D-Cys-ε-Lys 9-fold and 6-fold more efficiently than wt-mbPylRS respectively using GFP assays and immunoblotting using myoglobin-His6.
D-Cys-ε-Lys was incorporated into a T4 lysozyme using CysKRS, which was confirmed by ESI-MS.