RS/tRNA Foundational Publication Support
Wilkins, Bryan J, Samuel Marionni, Douglas D Young, Jia Liu, Yan Wang, Martino L Di Salvo, Alexander Deiters, and Ashton Cropp. (2010) 2010. “Site-Specific Incorporation Of Fluorotyrosines Into Proteins In Escherichia Coli By Photochemical Disguise.”. Biochemistry 49 (8): 1557-9. doi:10.1021/bi100013s.
RS/tRNA Pair Development Year
2010
ncAA(s) Incorporated
o-Nitrobenzyl-2-fluoro-L-tyrosine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Photo-caged fluoro-tyrosine
o-Nitrobenzyl-3 fluoro-L-tyrosine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
photocaged fluoroTyr
RS Organism of Origin
Parent RS
RS Mutations
T32G
L65G
H70N
F108G
D158S
I159M
L162N
L65G
H70N
F108G
D158S
I159M
L162N
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In DH10B cells, pTrc-HisA-sfGFP150TAG was transformed using ONB-2FYRS-1, with expression of 2mg/L full-length protein observed only in the presence of o-Nitrobenzyl-2-fluoro-L-tyrosine. Additionally, pTrc-HisA-sfGFP150TAG was transformed into DH10B cells using the RS in the presence of 1mM o-Nitrobenzyl-3-fluoro-L-tyrosine, which also yielded full-length protein only in the presence of ncAA.