RS/tRNA Foundational Publication Support
Chin, Jason W, Ashton Cropp, Christopher Anderson, Mridul Mukherji, Zhiwen Zhang, and Peter G Schultz. (2003) 2003. “An Expanded Eukaryotic Genetic Code.”. Science (New York, N.y.) 301 (5635): 964-7.
Hino, Nobumasa, Yuko Okazaki, Takatsugu Kobayashi, Akiko Hayashi, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2005) 2005. “Protein Photo-Cross-Linking In Mammalian Cells By Site-Specific Incorporation Of A Photoreactive Amino Acid.”. Nature Methods 2 (3): 201-6.
RS/tRNA Protocols and Structural Information
Hino, Nobumasa, Akiko Hayashi, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2006) 2006. “Site-Specific Incorporation Of Non-Natural Amino Acids Into Proteins In Mammalian Cells With An Expanded Genetic Code.”. Nature Protocols 1 (6): 2957-62.
Serfling, R, and I Coin. (2016) 2016. “Incorporation Of Unnatural Amino Acids Into Proteins Expressed In Mammalian Cells.”. Methods In Enzymology 580: 89-107. doi:10.1016/bs.mie.2016.05.003.
RS/tRNA Usage Publications
Wilkins, Bryan J, Nils Rall, Yogesh Ostwal, Tom Kruitwagen, Kyoko Hiragami-Hamada, Marco Winkler, Yves Barral, Wolfgang Fischle, and Heinz Neumann. (2014) 2014. “A Cascade Of Histone Modifications Induces Chromatin Condensation In Mitosis.”. Science (New York, N.y.) 343 (6166): 77-80. doi:10.1126/science.1244508.
Huang, Li-Yin, George Umanah, Melinda Hauser, Cagdas Son, Boris Arshava, Fred Naider, and Jeffrey M Becker. (2008) 2008. “Unnatural Amino Acid Replacement In A Yeast G Protein-Coupled Receptor In Its Native Environment.”. Biochemistry 47 (20): 5638-48. doi:10.1021/bi701866e.
RS/tRNA Pair Development Year
2003
ncAA(s) Incorporated
p-benzoyl-L-phenylalanine (Bpa)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Used as a photocrosslinker, allowing for crosslinking and bioorthogonal ligation of protein.
RS Organism of Origin
Parent RS
RS Mutations
Y37G
D182G
L186A
D182G
L186A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Multiple tRNAs?
Also shown in second foundational paper that this RS can work in CHO cells if one uses the B. stearothermophilus suppressor Tyr tRNA(CUA)
RS/tRNA Availability
n/a
Used in what cell line?
RS/tRNA Additional Notes
Bpa-containing hSOD(33) (human superoxide dismutase) was produced in yeast with full fidelity by MS analysis and at a yield said to be similar to the yield obtained for p-acetylPhe-SOD(33) using a different RS. That yield was ~50 ng/mL (corresponding to ~20% efficiency). A second selected RS designated p-benzoylPheRS-2 (w/ mutations Y37G,D182G, F183Y, L186M) was not further characterized.
Shown in second foundational paper to work in CHO cells using a B. stearothermophilus tRNA. And this system is described in the 2006 Nature Protocol.
Shown in second foundational paper to work in CHO cells using a B. stearothermophilus tRNA. And this system is described in the 2006 Nature Protocol.