RS/tRNA Foundational Publication Support
Chin, Jason W, Ashton Cropp, Christopher Anderson, Mridul Mukherji, Zhiwen Zhang, and Peter G Schultz. (2003) 2003. “An Expanded Eukaryotic Genetic Code.”. Science (New York, N.y.) 301 (5635): 964-7.
RS/tRNA Pair Development Year
2003
ncAA(s) Incorporated
p-iodo-L-phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Can be used for phasing in protein crystallography.
RS Organism of Origin
Parent RS
RS Mutations
Y37V
D182S
F183Y
D182S
F183Y
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
n/a
Used in what cell line?
RS/tRNA Additional Notes
The RS was shown in yeast to give high fidelity incorporation into hSOD(33). The paper also reported 2 other RSs from the same selection: p-iodoPhe-RS2 with mutations Y37I, D182S, F183M; and p-iodoPhe-RS3 with mutations Y37V, D182S, F183M, L186A.