RS/tRNA Foundational Publication Support
Wang, Jiangyun, Stefan M Schiller, and Peter G Schultz. (2007) 2007. “A Biosynthetic Route To Dehydroalanine-Containing Proteins.”. Angewandte Chemie (International Ed. In English) 46 (36): 6849-51.
RS/tRNA Pair Development Year
2007
ncAA(s) Incorporated
PhenylselenoCys
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Precursor of Dha
RS Organism of Origin
Parent RS
RS Mutations
Tyr32Trp
Leu65His
His70Gly
Phe108Asn
Gln109Ser
Asp158Ser
Leu162Glu
Leu65His
His70Gly
Phe108Asn
Gln109Ser
Asp158Ser
Leu162Glu
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In E. coli, plasmid pEt-GFPx242 was co-transformed alongside PhSeRS-K4 in the presence or absence of 1mM PhenylselenoCys. SDS-PAGE testing revealed full-length GFP expression solely in the presence of PhenylselenoCys with 5mg/L yield. Results were confirmed by ESI-MS.