RS/tRNA Foundational Publication Support
Miyake-Stoner, Shigeki J, Christian A Refakis, Jared T Hammill, Hrvoje Lusic, Jennifer L Hazen, Alexander Deiters, and Ryan A Mehl. (2010) 2010. “Generating Permissive Site-Specific Unnatural Aminoacyl-Trna Synthetases.”. Biochemistry 49 (8): 1667-77. doi:10.1021/bi901947r.
RS/tRNA Pair Development Year
2010
ncAA(s) Incorporated
Trifluoromethyl-L phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
19F Probe for protein
NMR
NMR
RS Organism of Origin
Parent RS
RS Mutations
Tyr32Val
A65V -> mutations from parent ptfmFRS
Phe108Trp
Gln109Met
S158A -> mutations from parent ptfmFRS
Ile159Met
A65V -> mutations from parent ptfmFRS
Phe108Trp
Gln109Met
S158A -> mutations from parent ptfmFRS
Ile159Met
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
https://www.addgene.org/85484/
Used in what cell line?
RS/tRNA Additional Notes
A65V and S158A mutations in the RS enabled highly permissive incorporation of 4-methylphenylalanine(pmF), 4-trifluoromethylphenylalanine(ptfmF), 3-fluoro-4-methylphenylalanine(pmmfF), and 3-fluoro-4-trifluoromethylphenylalanine(ptfmmfF) into sfGFP.