RS/tRNA Pair Development Year
2011
RS Organism of Origin
Parent RS
RS Mutations
none
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
C20U
RS/tRNA Availability
Addgene #34623 has the SepRS and EFSep, and Addgene #34626 has the Sep-tRNA and protein-of-interest genes
Used in what cell line?
RS/tRNA Additional Notes
This system does not achieve high fidelity pSer incorporation and has been superseded by GCE systems using SepRS2 (for protein production), and using SepRS9 (for studies of the human phosphoproteome expresssed in E. coli).