< To RS/tRNA Database

MbPylRS-WT

RS/tRNA Foundational Publication Support

Polycarpo, Carla R, Stephanie Herring, Amélie Bérubé, John L Wood, Dieter Söll, and Alexandre Ambrogelly. (2006) 2006. “Pyrrolysine Analogues As Substrates For Pyrrolysyl-Trna Synthetase.”. Febs Letters 580 (28-29): 6695-700.
Nguyen, Duy P, Hrvoje Lusic, Heinz Neumann, Prashant B Kapadnis, Alexander Deiters, and Jason W Chin. (2009) 2009. “Genetic Encoding And Labeling Of Aliphatic Azides And Alkynes In Recombinant Proteins Via A Pyrrolysyl-Trna Synthetase/Trna(Cua) Pair And Click Chemistry.”. Journal Of The American Chemical Society 131 (25): 8720-1. doi:10.1021/ja900553w.
Lang, Kathrin, Lloyd Davis, Jessica Torres-Kolbus, Chungjung Chou, Alexander Deiters, and Jason W Chin. (2012) 2012. “Genetically Encoded Norbornene Directs Site-Specific Cellular Protein Labelling Via A Rapid Bioorthogonal Reaction.”. Nature Chemistry 4 (4): 298-304. doi:10.1038/nchem.1250.

RS/tRNA Usage Publications

Lang, Kathrin, Lloyd Davis, Jessica Torres-Kolbus, Chungjung Chou, Alexander Deiters, and Jason W Chin. (2012) 2012. “Genetically Encoded Norbornene Directs Site-Specific Cellular Protein Labelling Via A Rapid Bioorthogonal Reaction.”. Nature Chemistry 4 (4): 298-304. doi:10.1038/nchem.1250.
RS/tRNA Pair Development Year
2006

ncAA(s) Incorporated

RS Organism of Origin
Methanosarcina barkeri
Parent RS
Pyl RS
RS Mutations
none
tRNA Organism of Origin
Methanosarcina barkeri
Parent tRNA
Pyl tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
DH10B
HEK293
RS/tRNA Additional Notes
Incorporation in E. coli into GFP position 150 E coli and also myoglobin and T4 lysozyme and confirmed by MS. ncAA-GFP produced at ~4 mg/L. In mammalian cells incorporated both norbornene and azide ncAAs into the EGF receptor and showed the norbornene reacted well with tetrazine reagent while the azide had minimal reaction with a cyclooctyne.